Mable (Wing Yan) Chan (MASc): The Role of Ex Vivo Polarized Monocyte/Macrophages in Osteoarthritis

November 29, 2017 @ 9:00 am – 9:30 am
Rosebrugh Building
Rosebrugh Bldg, Toronto, ON M5S 3G9

Room: RS 211


Immune cell infiltration and inflammation are key players in the progression of knee osteoarthritis (OA). The modulatory immune cells known as monocytes/macrophages have a spectrum of roles from inflammatory to homeostatic, acting as defenders against infection and mediators of wound healing. In OA, they are the most prevalent infiltrating inflammatory immune cell type. My project investigates the role of monocytes/macrophages in the OA joint and their contribution to joint inflammation and cartilage degradation. An in vitro joint explant model sourced from late stage OA cartilage and synovial tissue has been optimized for testing inflammatory and degradation response. Monocytes/macrophages were polarized using an ex vivo protocol into inflammatory “M1” and homeostatic “M2” phenotypes and co-cultured in the joint explants for 2-7 days. Gene expression and protein level results support a differential effect on joint tissue inflammation and degradation when comparing “M1” and “M2” treatment. This data supports further investigation of monocytes/macrophage cell therapy in OA as a treatment strategy.