Jonathan Rocheleau | Percy Edward Hart Professor in Biomedical Engineering & Associate Director, Research
BSc (Windsor), PhD (Western)
- Institute of Biomaterials & Biomedical Engineering
- Scientist, Toronto General Research Institute, University Health Network
- Department of Physiology
MaRS Discovery District
Princess Margaret Cancer Research Tower, 101 College Street, Room 10-361 (10th floor)
Toronto, Ontario, M5G 1L7 Canada
+1 416 581-7839 (office)
Quantitative Microscopy, Microfluidics and Metabolism (QuantM3) Laboratory (web)
IBBME Research Office
Rhonda Marley, Professional Programs & Research Office Assistant
+1 416 978-6102 (office)
Interaction between pancreatic islets and vascular endothelial cells is necessary for the maintenance of ß-cell mass and function. Aside from acting as a conduit for molecular oxygen, vascular endothelial cells in vivo secrete the majority of islet extracellular matrix (ECM). This ECM likely provides a permissive signal for ß-cell proliferation, contributing to the coordinated hyperplasia of these tissues during the early stages of Type 2 diabetes. This ECM also provides a reservoir for heparin binding growth factors that further modulate this hyperplasia, including fibroblast growth factor (FGF) and vascular endothelial growth factor-A (VEGF-A). We hypothesize that communication between ß-cells and vascular endothelial cells directs the proliferation and function of both tissues.
To examine ß-cell–vascular endothelial cell interaction, my lab uses a number of cutting-edge techniques: two-photon excitation microscopy, confocal microcopy, microfluidics, and live cell imaging of fluorescent proteins. Current projects use cell culture and ex vivo pancreatic islet models. These studies will advance our understanding of pancreatic islet communication, with a specific focus on the communication between ß-cell and vascular endothelial cells through FGF/FGFR1-signaling.
Keywords: Pancreatic islet, FGF/FGFR, Extracellular matrix, Two-photon microscopy, Confocal microscopy, Quantitative Live cell imaging
Autofluorescence imaging of living pancreatic islets reveals fibroblast growth factor-21 (FGF21)-induced metabolism.Sun MY, Yoo E, Green BJ, Altamentova SM, Kilkenny DM, Rocheleau JV. Biophysical Journal. 2012 Dec 5. 103(11):2379-2388.
Quantitative imaging of electron transfer flavoprotein autofluorescence reveals the dynamics of lipid partitioning in living pancreatic islets. Lam AK, Silva PN, Altamentova SM, Rocheleau JV. Integr Biol (Camb). 2012 Aug 23. 4(8):838-46.
The dynamics and distribution of Klothoβ (KLB) and Fibroblast growth factor receptor-1 (FGFR1) in living cells reveals the fibroblast growth factor-21 (FGF21)-induced receptor complex. Ming AYK, Yoo E, Vorontsov EN, Altamentova SM, Kilkenny DM, Rocheleau JV. The Journal of Biological Chemistry. June 8, 2012. Vol. 287, No. 24: pp. 19997–20006.
Culturing pancreatic islets in microfluidic flow enhances morphology of the associated endothelial cells. Sankar KS, Green BJ, Crocker AR, Verity JE, Altamentova SM, Rocheleau JV. PLoS One. 2011. 6(9):e24904.
Rapid extravasation and establishment of breast cancer micrometastases in the liver microenvironment. Martin MD, Kremers GJ, Short KW, Rocheleau JV, Xu L, Piston DW, Matrisian LM, Gorden DL. Mol Cancer Res. 2010 Oct. 8(10):1319-27.
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